The Adenine Nucleotide Translocase (ANT) family is a group of proteins located in the inner mitochondrial membrane, responsible for the transmembrane transport of adenine nucleotides (ADP/ATP). The main members include ANT1, ANT2, ANT3, and ANT4. They consist of multiple transmembrane helical domains and participate in cellular energy metabolism by exchanging ADP and ATP inside and outside the mitochondria through the formation of adenine nucleotide carriers. The expression of ANT family members is tissue- and cell-specific, and mutations or dysregulation of their genes are associated with various diseases, including cardiomyopathy, neurodegenerative diseases, and metabolic disorders.