Endogenous metabolites are compounds produced within an organism through natural metabolic processes, including amino acids, lipids, nucleotides, and carbohydrates, among other. These metabolites function in various cellular compartments such as the cytoplasm, mitochondria, and endoplasmic reticulum, participating in key biological processes such as energy generation, signal transduction, and maintenance of cellular structure. Gene expression and functional mutations directly influence the generation and regulation of metabolites, thereby affecting the organism's health status and disease progression. Research on endogenous metabolites provides insight into the metabolic state of organisms and the mechanisms of disease, offering valuable contributions to disease diagnosis, treatment, and prevention.